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Determination of phenylethanolamine N-methyltransferase activity in rat brain
Summary
Type:
HPLC
Application #:
102020
Catalog#:
720018.40 - MN HPLC Column, Nucleosil C18, 100A, 7um, 4x250mm
Detection:
fluorescence
Conditions:
eluent: 0.05M Na phosphate buffer pH 2.6 containing 2mM Na n-heptanesulphonate and 1% acetonitrile,
flow rate 0.8ml/min
Authors:
Trocewicz J
Source:
J Chromatogr 233 (1982) 328 - 333
Details
Legend: Chromatograms of PNMT incubation mixtures, A) 1h incubation at 37°C with a homogenizate of 10mg of rat pons plus medulla oblongata as enzyme, B) blank incubation without enzyme, C) another blank
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